Traceless aptamer-mediated isolation of CD8⁺ T cells for chimeric antigen receptor T-cell therapy.

ABSTRACT: Chimeric antigen receptor T-cell therapies using defined product compositions require high-purity T-cell isolation systems that, unlike immunomagnetic positive enrichment, are inexpensive and leave no trace on the final cell product. Here, we show that DNA aptamers (generated with a modified cell-SELEX procedure to display low-nanomolar affinity for the T-cell marker CD8) enable the traceless isolation of pure CD8⁺ T cells at low cost and high yield. Captured CD8⁺ T cells are released label-free by complementary oligonucleotides that undergo toehold-mediated strand displacement with the aptamer. We also show that chimeric antigen receptor T cells manufactured from these cells are comparable to antibody-isolated chimeric antigen receptor T cells in proliferation, pheno

SUBMITTER: Kacherovsky N 

PROVIDER: S-EPMC6783348 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature