IgM, IgG, and IgA Influenza-Specific Plasma Cells Express Divergent Transcriptomes.

ABSTRACT: Ab-secreting cells (ASC) or plasma cells are essential components of the humoral immune system. Although Abs of different isotypes have distinct functions, it is not known if the ASC that secrete each isotype are also distinct. ASC downregulate their surface BCR upon differentiation, hindering analyses that couple BCR information to other molecular characteristics. In this study, we developed a methodology using fixation, permeabilization, and intracellular staining coupled with cell sorting and reversal of the cross-links to allow RNA sequencing of isolated cell subsets. Using hemagglutinin and nucleoprotein Ag-specific B cell tetramers and intracellular staining for IgM, IgG, and IgA isotypes, we were able to derive and compare the gene expression programs of ASC subsets that were respon

SUBMITTER: Price MJ 

PROVIDER: S-EPMC6783370 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature