Electro-optic imaging enables efficient wide-field fluorescence lifetime microscopy.

ABSTRACT: Nanosecond temporal resolution enables new methods for wide-field imaging like time-of-flight, gated detection, and fluorescence lifetime. The optical efficiency of existing approaches, however, presents challenges for low-light applications common to fluorescence microscopy and single-molecule imaging. We demonstrate the use of Pockels cells for wide-field image gating with nanosecond temporal resolution and high photon collection efficiency. Two temporal frames are obtained by combining a Pockels cell with a pair of polarizing beam-splitters. We show multi-label fluorescence lifetime imaging microscopy (FLIM), single-molecule lifetime spectroscopy, and fast single-frame FLIM at the camera frame rate with 10³-10⁵ times higher throughput than single photon counting. F

SUBMITTER: Bowman AJ 

PROVIDER: S-EPMC6783475 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature