Definition of the Minimal Contents for the Molecular Simulation of the Yeast Cytoplasm.

ABSTRACT: The cytoplasm is a densely packed environment filled with macromolecules with hindered diffusion. Molecular simulation of the diffusion of biomolecules under such macromolecular crowding conditions requires the definition of a simulation cell with a cytoplasmic-like composition. This has been previously done for prokaryote cells (E. coli) but not for eukaryote cells such as yeast as a model organism. Yeast proteomics datasets vary widely in terms of cell growth conditions, the technique used to determine protein composition, the reported relative abundance of proteins, and the units in which abundances are reported. We determined that the gene ontology profiles of the most abundant proteins across these datasets are similar, but their abundances vary greatly. To overcome this proble

SUBMITTER: Kompella VPS 

PROVIDER: S-EPMC6783697 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature