Genomic Analysis of <i>Clostridium perfringens</i> BEC/CPILE-Positive, Toxinotype D and E Strains Isolated from Healthy Children.

ABSTRACT: Clostridium perfringens toxinotype D, toxinotype E, and gastroenteritis-linked BEC/CPILE-positive strains have never been reported in healthy children. We isolated, whole-genome sequenced and bioinformatically characterised three C. perfringens isolates-type D (IQ1), type E (IQ2) and BEC/CPILE-positive (IQ3), recovered from the stools of three healthy two-year-olds, which were further compared to 128 C. perfringens genomes available from NCBI. The analysis uncovered a previously under-described putative toxin gene alv (alveolysin) encoded by isolates IQ2 and IQ3, which appeared to be a clade-specific trait associated with strains from domestic animals. A plasmid analysis indicated that the iota-toxin was encoded on a near-intact previously described plasmid pCPP

SUBMITTER: Kiu R 

PROVIDER: S-EPMC6783817 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature