Crystal Structure of African Swine Fever Virus A179L with the Autophagy Regulator Beclin.

ABSTRACT: Subversion of programmed cell death-based host defence systems is a prominent feature of infections by large DNA viruses. African swine fever virus (ASFV) is a large DNA virus and sole member of the Asfarviridae family that harbours the B-cell lymphoma 2 or Bcl-2 homolog A179L. A179L has been shown to bind to a range of cell death-inducing host proteins, including pro-apoptotic Bcl-2 proteins as well as the autophagy regulator Beclin. Here we report the crystal structure of A179L bound to the Beclin BH3 motif. A179L engages Beclin using the same canonical ligand-binding groove that is utilized to bind to pro-apoptotic Bcl-2 proteins. The mode of binding of Beclin to A179L mirrors that of Beclin binding to human Bcl-2 and Bcl-x_L as well as murine γ-herpesvirus 68. The intr

SUBMITTER: Banjara S 

PROVIDER: S-EPMC6784060 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature