The Dynamics of the Cell Wall Proteome of Developing Alfalfa Stems.

ABSTRACT: In this study, the cell-wall-enriched subproteomes at three different heights of alfalfa stems were compared. Since these three heights correspond to different states in stem development, a view on the dynamics of the cell wall proteome during cell maturation is obtained. This study of cell wall protein-enriched fractions forms the basis for a description of the development process of the cell wall and the linking cell wall localized proteins with the evolution of cell wall composition and structure. The sequential extraction of cell wall proteins with CaCl₂, EGTA, and LiCl-complemented buffers was combined with a gel-based proteome approach and multivariate analysis. Although the highest similarities were observed between the apical and intermediate stem regions, the proteome p

SUBMITTER: Sergeant K 

PROVIDER: S-EPMC6784106 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature