Pulmonary Metagenomic Sequencing Suggests Missed Infections in Immunocompromised Children.

ABSTRACT:

Background

Despite improved diagnostics, pulmonary pathogens in immunocompromised children frequently evade detection, leading to significant mortality. Therefore, we aimed to develop a highly sensitive metagenomic next-generation sequencing (mNGS) assay capable of evaluating the pulmonary microbiome and identifying diverse pathogens in the lungs of immunocompromised children.

Methods

We collected 41 lower respiratory specimens from 34 immunocompromised children undergoing evaluation for pulmonary disease at 3 children's hospitals from 2014-2016. Samples underwent mechanical homogenization, parallel RNA/DNA extraction, and metagenomic sequencing. Sequencing reads were aligned to the National Center for Biotechnology Information nucleotide reference database to determine taxo

SUBMITTER: Zinter MS 

PROVIDER: S-EPMC6784263 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature