CRISPR-Cas9 induces point mutation in the mucormycosis fungus Rhizopus delemar.

ABSTRACT: Rhizopus delemar causes devastating mucormycosis in immunodeficient individuals. Despite its medical importance, R. delemar remains understudied largely due to the lack of available genetic markers, the presence of multiple gene copies due to genome duplication, and mitotically unstable transformants resulting from conventional and limited genetic approaches. The clustered regularly interspaced short palindromic repeat (CRISPR)-associated nuclease 9 (Cas9) system induces efficient homologous and non-homologous break points and generates individual and multiple mutant alleles without requiring selective marker genes in a wide variety of organisms including fungi. Here, we have successfully adapted this technology for inducing gene-specific single nucleotide (nt) deletions in two clinical st

SUBMITTER: Bruni GO 

PROVIDER: S-EPMC6784326 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature