Impact of Hydrogel Stiffness on Differentiation of Human Adipose-Derived Stem Cell Microspheroids.

ABSTRACT: Hydrogels represent an attractive material platform for realization of three-dimensional (3D) tissue-engineered constructs, as they have tunable mechanical properties, are compatible with different types of cells, and resemble elements found in natural extracellular matrices. So far, numerous hydrogel-cartilage/bone tissue engineering (TE)-related studies were performed by utilizing a single cell encapsulation approach. Although multicellular spheroid cultures exhibit advantageous properties for cartilage or bone TE, the chondrogenic or osteogenic differentiation potential of stem cell microspheroids within hydrogels has not been investigated much. This study explores, for the first time, how stiffness of gelatin-based hydrogels (having a storage modulus of 538, 3584, or 7263 Pa) affects p

SUBMITTER: Zigon-Branc S 

PROVIDER: S-EPMC6784494 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature