Developing a low-cost milliliter-scale chemostat array for precise control of cellular growth.

ABSTRACT:

Background

Multiplexed milliliter-scale chemostats are useful for measuring cell physiology under various degrees of nutrient limitation and for carrying out evolution experiments. In each chemostat, fresh medium containing a growth rate-limiting metabolite is pumped into the culturing chamber at a constant rate, while culture effluent exits at an equal rate. Although such devices have been developed by various labs, key parameters - the accuracy, precision, and operational range of flow rate - are not explicitly characterized.

Methods

Here we re-purpose a published multiplexed culturing device to develop a multiplexed milliliter-scale chemostat. Flow rates for eight chambers can be independently controlled to a wide range, corresponding to population doubling times of 3~13

SUBMITTER: Skelding D 

PROVIDER: S-EPMC6785052 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature