Associations between Maternal Tobacco Smoke Exposure and the Cord Blood CD 4 + DNA Methylome.

ABSTRACT:

Background

Maternal tobacco smoke exposure has been associated with altered DNA methylation. However, previous studies largely used methylation arrays, which cover a small fraction of CpGs, and focused on whole cord blood.

Objectives

The current study examined the impact of in utero exposure to maternal tobacco smoke on the cord blood CD4+ DNA methylome.

Methods

The methylomes of 20 Hispanic white newborns (n=10 exposed to any maternal tobacco smoke in pregnancy; n=10 unexposed) from the Maternal and Child Health Study (MACHS) were profiled by whole-genome bisulfite sequencing (median coverage: 6.5×). Statistical analyses were conducted using the Regression Analysis of Differential Methylation (RADMeth) program because it performs well on low-coverage data (minimizes

SUBMITTER: Howe CG 

PROVIDER: S-EPMC6785223 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature