Production of cutinase from Fusarium falciforme and its application for hydrophilicity improvement of polyethylene terephthalate fabric.

ABSTRACT: Among 23 isolates of cutinase-producing fungi from Thailand, one strain of Fusarium falciforme PBURU-T5 exhibited the greatest cutinase activity (3.36 ± 0.12 U ml^-1) against p-nitrophenyl butyrate. This strain was found to produce an inducible cutinase when cultivated in the liquid mineral medium containing cutin from papaya peel as the sole carbon source. By optimizing the production condition based on the central composite experimental design, the maximal cutinase activity up to 4.82 ± 0.18 U ml^-1 was attained under the condition: 0.4% (w/v) papaya cutin as the carbon source, 0.3% (w/v) peptone as the nitrogen source, incubation temperature at 30 °C for 4 days, and initial pH 7.0. The crude enzyme was optimally active at 35 °C and pH 9.0 which was suit

SUBMITTER: Sooksai T 

PROVIDER: S-EPMC6785599 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature