Establishment of an efficient reverse genetic system of Mumps virus S79 from cloned DNA.

ABSTRACT:

Background

Mumps is a common type of respiratory infectious disease caused by mumps virus (MuV), and can be effectively prevented by vaccination. In this study, a reverse genetic system of MuV that can facilitate the rational design of safer, more efficient mumps vaccine candidates is established.

Methods

MuV-S79 cDNA clone was assembled into a full-length plasmid by means of the GeneArt™ High-Order Genetic Assembly System, and was rescued via reverse genetic technology. RT-PCR, sequencing, and immunofluorescence assays were used for rMuV-S79 authentication. Viral replication kinetics and in vivo experimental models were used to evaluate the replication, safety, and immunogenicity of rMuV-S79.

Results

A full-length cDNA clone of MuV-S79 in the assembly process was gen

SUBMITTER: Zhou D 

PROVIDER: S-EPMC6785654 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature