⁶⁸Ga-DOTA-E[c(RGDfK)]₂ PET Imaging of SHARPIN-Regulated Integrin Activity in Mice.

ABSTRACT: Shank-associated RH domain-interacting protein (SHARPIN) is a cytosolic protein that plays a key role in activation of nuclear factor κ-light-chain enhancer of activated B cells and regulation of inflammation. Furthermore, SHARPIN controls integrin-dependent cell adhesion and migration in several normal and malignant cell types, and loss of SHARPIN correlates with increased integrin activity in mice. Arginyl-glycyl-aspartic acid (RGD), a cell adhesion tripeptide motif, is an integrin recognition sequence that facilitates PET imaging of integrin upregulation during tumor angiogenesis. We hypothesized that increased integrin activity due to loss of SHARPIN protein would affect the uptake of α_vβ₃-selective cyclic, dimeric peptide ⁶⁸Ga-DOTA-E[c(RGDfK)]₂

SUBMITTER: Siitonen R 

PROVIDER: S-EPMC6785786 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature