Tumor Imaging Using Radiolabeled Matrix Metalloproteinase-Activated Anthrax Proteins.

ABSTRACT: Increased activity of matrix metalloproteinases (MMPs) is associated with worse prognosis in different cancer types. The wild-type protective antigen (PA-WT) of the binary anthrax lethal toxin was modified to form a pore in cell membranes only when cleaved by MMPs (to form PA-L1). Anthrax lethal factor (LF) is then able to translocate through these pores. Here, we used a ¹¹¹In-radiolabeled form of LF with the PA/LF system for noninvasive in vivo imaging of MMP activity in tumor tissue by SPECT. Methods: MMP-mediated activation of PA-L1 was correlated to anthrax receptor expression and MMP activity in a panel of cancer cells (HT1080, MDA-MB-231, B8484, and MCF7). Uptake of ¹¹¹In-radiolabeled PA-L1, ¹¹¹In-PA-WT^K563C, or ¹¹¹In-LF

SUBMITTER: Elvina Xavier MA 

PROVIDER: S-EPMC6785798 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature