Metabolic perturbations in mutants of glucose transporters and their applications in metabolite production in Escherichia coli.

ABSTRACT:

Background

Most microorganisms have evolved to maximize growth rate, with rapid consumption of carbon sources from the surroundings. However, fast growing phenotypes usually feature secretion of organic compounds. For example, E. coli mainly produced acetate in fast growing condition such as glucose rich and aerobic condition, which is troublesome for metabolic engineering because acetate causes acidification of surroundings, growth inhibition and decline of production yield. The overflow metabolism can be alleviated by reducing glucose uptake rate.

Results

As glucose transporters or their subunits were knocked out in E. coli, the growth and glucose uptake rates decreased and biomass yield was improved. Alteration of intracellular metabolism caused by the mutations was inves

SUBMITTER: Jung HM 

PROVIDER: S-EPMC6786474 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature