Bright sub-20-nm cathodoluminescent nanoprobes for electron microscopy.

ABSTRACT: Electron microscopy has been instrumental in our understanding of complex biological systems. Although electron microscopy reveals cellular morphology with nanoscale resolution, it does not provide information on the location of different types of proteins. An electron-microscopy-based bioimaging technology capable of localizing individual proteins and resolving protein-protein interactions with respect to cellular ultrastructure would provide important insights into the molecular biology of a cell. Here, we synthesize small lanthanide-doped nanoparticles and measure the absolute photon emission rate of individual nanoparticles resulting from a given electron excitation flux (cathodoluminescence). Our results suggest that the optimization of nanoparticle composition, synthesis protocols an

SUBMITTER: Prigozhin MB 

PROVIDER: S-EPMC6786485 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature