Gene expression profiling of whole blood: A comparative assessment of RNA-stabilizing collection methods.

ABSTRACT: Peripheral Blood gene expression is widely used in the discovery of biomarkers and development of therapeutics. Recently, a spate of commercial blood collection and preservation systems have been introduced with proprietary variations that may differentially impact the transcriptomic profiles. Comparative analysis of these collection platforms will help optimize protocols to detect, identify, and reproducibly validate true biological variance among subjects. In the current study, we tested two recently introduced whole blood collection methods, RNAgard® and PAXgene® RNA, in addition to the traditional method of peripheral blood mononuclear cells (PBMCs) separated from whole blood and preserved in Trizol reagent. Study results revealed striking differences in the transcriptomic profiles fro

SUBMITTER: Donohue DE 

PROVIDER: S-EPMC6786555 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature