Augmentation of T-Cell Activation by Oscillatory Forces and Engineered Antigen-Presenting Cells.

ABSTRACT: Activation of T cells by antigen presenting cells (APCs) initiates their proliferation, cytokine production, and killing of infected or cancerous cells. We and others have shown that T-cell receptors require mechanical forces for triggering, and these forces arise during the interaction of T cells with APCs. Efficient activation of T cells in vitro is necessary for clinical applications. In this paper, we studied the impact of combining mechanical, oscillatory movements provided by an orbital shaker with soft, biocompatible, artificial APCs (aAPCs) of various sizes and amounts of antigen. We showed that these aAPCs allow for testing the strength of signal delivered to T cells, and enabled us to confirm that that absolute amounts of antigen engaged by the T cell are more important for activ

SUBMITTER: Majedi FS 

PROVIDER: S-EPMC6786928 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature