Meiotic crossovers characterized by haplotype-specific chromosome painting in maize.

ABSTRACT: Meiotic crossovers (COs) play a critical role in generating genetic variation and maintaining faithful segregation of homologous chromosomes during meiosis. We develop a haplotype-specific fluorescence in situ hybridization (FISH) technique that allows visualization of COs directly on metaphase chromosomes. Oligonucleotides (oligos) specific to chromosome 10 of maize inbreds B73 and Mo17, respectively, are synthesized and labeled as FISH probes. The parental and recombinant chromosome 10 in B73 x Mo17 F₁ hybrids and F₂ progenies can be unambiguously identified by haplotype-specific FISH. Analysis of 58 F₂ plants reveals lack of COs in the entire proximal half of chromosome 10. However, we detect COs located in regions very close to the centromere in recombi

SUBMITTER: do Vale Martins L 

PROVIDER: S-EPMC6787048 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature