DNA enrichment and tagmentation method for species-level identification and strain-level differentiation using ON-rep-seq.

ABSTRACT: Despite the massive developments within culture-independent methods for detection of microorganisms during the last decade, culture-based methods remain a cornerstone in microbiology. Yet, the problem of rapid, accurate and inexpensive identification of bacterial isolates down to species/strain level remains unresolved. We have developed a new method for bacterial DNA enrichment and tagmentation allowing fast (<24 h) and cost-effective species level identification and strain level differentiation using the MinION portable sequencing platform (ON-rep-seq). DNA library preparation for 96 isolates takes less than 5 h and ensures highly reproducible distribution of reads that can be used to generate strain level specific read length counts profiles (LCp). We have developed a pipeline that by c

SUBMITTER: Krych L 

PROVIDER: S-EPMC6787052 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature