Three-Dimensional Microscopy by Milling with Ultraviolet Excitation.

ABSTRACT: Analysis of three-dimensional biological samples is critical to understanding tissue function and the mechanisms of disease. Many chronic conditions, like neurodegenerative diseases and cancers, correlate with complex tissue changes that are difficult to explore using two-dimensional histology. While three-dimensional techniques such as confocal and light-sheet microscopy are well-established, they are time consuming, require expensive instrumentation, and are limited to small tissue volumes. Three-dimensional microscopy is therefore impractical in clinical settings and often limited to core facilities at major research institutions. There would be a tremendous benefit to providing clinicians and researchers with the ability to routinely image large three-dimensional tissue volumes at cell

SUBMITTER: Guo J 

PROVIDER: S-EPMC6787072 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature