Real-time transfer of lentiviral particles by producer cells using an engineered coculture system.

ABSTRACT: Lentiviruses are quite effective gene delivery systems for stable production of genetically engineered human cells. However, prior to using lentivirus to deliver genetic materials to cells of interest, the normal course of production of these lentiviruses involves a lengthy collection, purification, preservation, and quantification process. In this report, we demonstrate the ability for producer HEK293T cells to simultaneously produce lentiviral particles and transduce (i.e., infect) target cells through a membrane-based coculture system in a continuous, real-time mode which negates the need for a separate viral collection and quantification process. The coculture system was evaluated for major design features such as variations in HEK293T seeding density, target cell type densities, as we

SUBMITTER: Timmins LM 

PROVIDER: S-EPMC6787137 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature