Using DNA Metabarcoding To Evaluate the Plant Component of Human Diets: a Proof of Concept.

ABSTRACT: Dietary intake is difficult to measure reliably in humans because approaches typically rely on self-reporting, which can be incomplete and biased. In field studies of animals, DNA sequencing-based approaches such as metabarcoding have been developed to characterize diets, but such approaches have not previously been widely applied to humans. Here, we present data derived from sequencing of a chloroplast DNA marker (the P6 loop of the trnL [UAA] intron) in stool samples collected from 11 individuals consuming both controlled and freely selected diets. The DNA metabarcoding strategy resulted in successful PCR amplification in about 50% of samples, which increased to a 70% success rate in samples from individuals eating a controlled plant-rich diet. Detection of plant taxa among sequen

SUBMITTER: Reese AT 

PROVIDER: S-EPMC6787566 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature