Blood Vessel Patterning on Retinal Astrocytes Requires Endothelial Flt-1 (VEGFR-1).

ABSTRACT: Feedback mechanisms are critical components of many pro-angiogenic signaling pathways that keep vessel growth within a functional range. The Vascular Endothelial Growth Factor-A (VEGF-A) pathway utilizes the decoy VEGF-A receptor Flt-1 to provide negative feedback regulation of VEGF-A signaling. In this study, we investigated how the genetic loss of flt-1 differentially affects the branching complexity of vascular networks in tissues despite similar effects on endothelial sprouting. We selectively ablated flt-1 in the post-natal retina and found that maximum induction of flt-1 loss resulted in alterations in endothelial sprouting and filopodial extension, ultimately yielding hyper-branched networks in the absence of changes in retinal astrocyte architecture. The mosaic deletion of flt-1 re

SUBMITTER: Chappell JC 

PROVIDER: S-EPMC6787756 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature