Quantitative iTRAQ-based proteomic analysis of differentially expressed proteins in aging in human and monkey.

ABSTRACT:

Background

The underlying physiological mechanisms associated with aging are still complex and unclear. As a very important tissue of human body, the circulatory system also plays a very important role in the process of aging. In this study, we use the isobaric tags for relative and absolute quantification (iTRAQ) method to identify differentially expressed proteins in plasma for humans and monkeys between young and aged. Western blotting and behavioral experiment in mice were performed to validate the expression of the candidate protein.

Results

Between the young / the old humans and the young / the old monkeys 74 and 69 proteins were found to be differently expressed, respectively. For the human samples, these included 38 up-regulated proteins and 36 down-regulated protein

SUBMITTER: Wang H 

PROVIDER: S-EPMC6788010 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature