Novel circular RNAs expressed in brain microvascular endothelial cells after oxygen-glucose deprivation/recovery.

ABSTRACT: Circular RNAs (circRNAs) are generated by head-to-tail splicing and are ubiquitously expressed in all multicellular organisms. Their important biological functions are increasingly recognized. Cerebral ischemia reperfusion injury-induced brain microvascular endothelial cell dysfunction is an initial stage of blood-brain barrier disruption. The expression profile and potential function of circRNAs in brain microvascular endothelial cells is unknown. Rat brain microvascular endothelial cells were extracted and cultured in glucose-free medium for 4 hours with 5% CO₂ and 95% N₂, and the medium was then replaced with complete growth medium for 6 hours. The RNA in these cells was then extracted. The circRNA was identified by Find_circ and CIRI2 software. Functional and path

SUBMITTER: Liu W 

PROVIDER: S-EPMC6788225 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature