Lentiviral-based reporter constructs for profiling chondrogenic activity in primary equine cell populations.

ABSTRACT: Successful clinical translation of mesenchymal stem cell (MSC)-based therapies for cartilage repair will likely require the implementation of standardised protocols and broadly applicable tools to facilitate the comparisons among cell types and chondroinduction methods. The present study investigated the utility of recombinant lentiviral reporter vectors as reliable tools for comparing chondrogenic potential among primary cell populations and distinguishing cellular-level variations of chondrogenic activity in widely used three-dimensional (3D) culture systems. Primary equine MSCs and chondrocytes were transduced with vectors containing combinations of fluorescent and luciferase reporter genes under constitutive cytomeglavirus (CMV) or chondrocyte-lineage (Col2) promoters. Reporter activit

SUBMITTER: Martin-Pena A 

PROVIDER: S-EPMC6788286 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature