Dickkopf-4 gene expression is associated with differentiation and lymph node metastasis in colorectal cancer.

ABSTRACT:

Background and aim

Although high expression of Dickkopf-4 (DKK-4) in colorectal cancer has been reported in previous studies, its impact on clinicopathological features, including the prognosis and mechanism of expression, has not been well clarified to date.

Methods

(i) DKK-4 protein expression was analyzed by immunohistochemical staining with anti-DKK-4 antibody using archived formalin-fixed paraffin-embedded specimens obtained at surgery from 122 patients with colorectal cancer, and its association with clinicopathological findings was also investigated. (ii) The association between intratumoral DKK-4 protein expression and somatic hotspot mutations in cancer-associated genes in 40 patients was investigated using a next-generation sequencer.

Results

In cross-sectio

SUBMITTER: Tsukui Y 

PROVIDER: S-EPMC6788377 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature