Imaging Distal Aqueous Outflow Pathways in a Spontaneous Model of Congenital Glaucoma.

ABSTRACT:

Purpose

To validate the use of aqueous angiography (AA) in characterizing distal aqueous outflow pathways in normal and glaucomatous cats.

Methods

Ex vivo AA and optical coherence tomography (OCT) were performed in nine adult cat eyes (5 feline congenital glaucoma [FCG] and 4 normal), following intracameral infusion of 2.5% fluorescein and/or 0.4% indocyanine green (ICG) at physiologic intraocular pressure (IOP). Scleral OCT line scans were acquired in areas of high- and low-angiographic signal. Tissues dissected in regions of high- and low-AA signal, were sectioned and hematoxylin and eosin (H&E)-stained or immunolabeled (IF) for vascular endothelial and perivascular cell markers. Outflow vessel numbers and locations were compared between groups by Student's t-test.<

SUBMITTER: Snyder KC 

PROVIDER: S-EPMC6788461 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature