Seconds-resolved pharmacokinetic measurements of the chemotherapeutic irinotecan in situ in the living body.

ABSTRACT: The ability to measure drugs in the body rapidly and in real time would advance both our understanding of pharmacokinetics and our ability to optimally dose and deliver pharmacological therapies. To this end, we are developing electrochemical aptamer-based (E-AB) sensors, a seconds-resolved platform technology that, as critical for performing measurements in vivo, is reagentless, reversible, and selective enough to work when placed directly in bodily fluids. Here we describe the development of an E-AB sensor against irinotecan, a member of the camptothecin family of cancer chemotherapeutics, and its adaptation to in vivo sensing. To achieve this we first re-engineered (via truncation) a previously reported DNA aptamer against the camptothecins to support high-gain E-AB

SUBMITTER: Idili A 

PROVIDER: S-EPMC6788505 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature