Occurrence and stability of hetero-hexamer associations formed by β-carboxysome CcmK shell components.

ABSTRACT: The carboxysome is a bacterial micro-compartment (BMC) subtype that encapsulates enzymatic activities necessary for carbon fixation. Carboxysome shells are composed of a relatively complex cocktail of proteins, their precise number and identity being species dependent. Shell components can be classified in two structural families, the most abundant class associating as hexamers (BMC-H) that are supposed to be major players for regulating shell permeability. Up to recently, these proteins were proposed to associate as homo-oligomers. Genomic data, however, demonstrated the existence of paralogs coding for multiple shell subunits. Here, we studied cross-association compatibilities among BMC-H CcmK proteins of Synechocystis sp. PCC6803. Co-expression in Escherichia coli proved a consistent fo

SUBMITTER: Garcia-Alles LF 

PROVIDER: S-EPMC6788708 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature