Acetyl-CoA-mediated activation of Mycobacterium tuberculosis isocitrate lyase 2.

ABSTRACT: Isocitrate lyase is important for lipid utilisation by Mycobacterium tuberculosis but its ICL2 isoform is poorly understood. Here we report that binding of the lipid metabolites acetyl-CoA or propionyl-CoA to ICL2 induces a striking structural rearrangement, substantially increasing isocitrate lyase and methylisocitrate lyase activities. Thus, ICL2 plays a pivotal role regulating carbon flux between the tricarboxylic acid (TCA) cycle, glyoxylate shunt and methylcitrate cycle at high lipid concentrations, a mechanism essential for bacterial growth and virulence.

SUBMITTER: Bhusal RP 

PROVIDER: S-EPMC6788997 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature