Proteomic atlas of organ vasculopathies triggered by Staphylococcus aureus sepsis.

ABSTRACT: Sepsis is a life-threatening condition triggered by a dysregulated host response to microbial infection resulting in vascular dysfunction, organ failure and death. Here we provide a semi-quantitative atlas of the murine vascular cell-surface proteome at the organ level, and how it changes during sepsis. Using in vivo chemical labeling and high-resolution mass spectrometry, we demonstrate the presence of a vascular proteome that is perfusable and shared across multiple organs. This proteome is enriched in membrane-anchored proteins, including multiple regulators of endothelial barrier functions and innate immunity. Further, we automated our workflows and applied them to a murine model of methicillin-resistant Staphylococcus aureus (MRSA) sepsis to unravel changes during systemic inflammator

SUBMITTER: Toledo AG 

PROVIDER: S-EPMC6789120 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature