Network Analysis of a Membrane-Enriched Brain Proteome across Stages of Alzheimer's Disease.

ABSTRACT: Previous systems-based proteomic approaches have characterized alterations in protein co-expression networks of unfractionated asymptomatic (AsymAD) and symptomatic Alzheimer's disease (AD) brains. However, it remains unclear how sample fractionation and sub-proteomic analysis influences the organization of these protein networks and their relationship to clinicopathological traits of disease. In this proof-of-concept study, we performed a systems-based sub-proteomic analysis of membrane-enriched post-mortem brain samples from pathology-free control, AsymAD, and AD brains (n = 6 per group). Label-free mass spectrometry based on peptide ion intensity was used to quantify the 18 membrane-enriched fractions. Differential expression and weighted protein co-expression network analysis (W

SUBMITTER: Higginbotham L 

PROVIDER: S-EPMC6789842 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature