Proteomic Analysis of Antigen 60 Complex of M. bovis Bacillus Calmette-Guerin Reveals Presence of Extracellular Vesicle Proteins and Predicted Functional Interactions.

ABSTRACT: Tuberculosis (TB) is ranked among the top 10 causes of death worldwide. New biomarker-based serodiagnostics and vaccines are unmet needs stalling disease control. Antigen 60 (A60) is a thermostable mycobacterial complex typically purified from Bacillus Calmette-Guérin (BCG) vaccine. A60 was historically evaluated for TB serodiagnostic and vaccine potential with variable findings. Despite containing immunogenic proteins, A60 has yet to be proteomically characterized. Here, commercial A60 was (1) trypsin-digested in-solution, analyzed by LC-MS/MS, searched against M. tuberculosis H37Rv and M. bovis BCG Uniprot databases; (2) analyzed using STRING to predict protein-protein interactions; and (3) probed with anti-TB monoclonal antibodies and patient immunoglobulin G (IgG) on West

SUBMITTER: Hanafiah KM 

PROVIDER: S-EPMC6789874 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature