PK-M2-mediated metabolic changes in breast cancer cells induced by ionizing radiation.

ABSTRACT:

Purpose

Radiotherapy (RT) constitutes an important part of breast cancer treatment. However, triple negative breast cancers (TNBC) exhibit remarkable resistance to most therapies, including RT. Developing new ways to radiosensitize TNBC cells could result in improved patient outcomes. The M2 isoform of pyruvate kinase (PK-M2) is believed to be responsible for the re-wiring of cancer cell metabolism after oxidative stress. The aim of the study was to determine the effect of ionizing radiation (IR) on PK-M2-mediated metabolic changes in TNBC cells, and their survival. In addition, we determine the effect of PK-M2 activators on breast cancer stem cells, a radioresistant subpopulation of breast cancer stem cells.

Methods

Glucose uptake, lactate production, and glutamine consumpt

SUBMITTER: Zhang L 

PROVIDER: S-EPMC6790295 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature