CRISPR/Cas9-mediated functional recovery of the recessive rc allele to develop red rice.

ABSTRACT: Red rice contains high levels of proanthocyanidins and anthocyanins, which have been recognized as health-promoting nutrients. The red coloration of rice grains is controlled by two complementary genes, Rc and Rd. The RcRd genotype produces red pericarp in wild species Oryza rufipogon, whereas most cultivated rice varieties produce white grains resulted from a 14-bp frame-shift deletion in the seventh exon of the Rc gene. In the present study, we developed a CRISPR/Cas9-mediated method to functionally restore the recessive rc allele through reverting the 14-bp frame-shift deletion to in-frame mutations in which the deletions were in multiples of three bases, and successfully converted three elite white pericarp rice varieties into red ones. Rice seeds from T₁ in-frame Rc lines w

SUBMITTER: Zhu Y 

PROVIDER: S-EPMC6790373 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature