A transferase interactome that may facilitate channeling of polyunsaturated fatty acid moieties from phosphatidylcholine to triacylglycerol.

ABSTRACT: Polyunsaturated fatty acids (PUFAs) such as α-linolenic acid (ALA, 18:3Δ^{9 cis ,12 cis ,15 cis} ) have high nutritional and industrial values. In oilseed crops, PUFAs are synthesized on phosphatidylcholine (PC) and accumulated in triacylglycerol (TAG). Therefore, exploring the mechanisms that route PC-derived PUFA to TAG is essential for understanding and improving PUFA production. The seed oil of flax (Linum usitatissimum) is enriched in ALA, and this plant has many lipid biosynthetic enzymes that prefer ALA-containing substrates. In this study, using membrane yeast two-hybrid and bimolecular fluorescence complementation assays, we probed recombinant flax transferase enzymes, previously shown to co

SUBMITTER: Xu Y 

PROVIDER: S-EPMC6791306 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature