Reduced Myogenic and Increased Adipogenic Differentiation Capacity of Rotator Cuff Muscle Stem Cells.

ABSTRACT: BACKGROUND:Fat accumulation commonly occurs in chronically torn rotator cuff muscles, and increased fat within the rotator cuff is correlated with poor clinical outcomes. The extent of lipid deposition is particularly pronounced in injured rotator cuff muscles compared with other commonly injured muscles such as the gastrocnemius. Satellite cells, which are a tissue-resident muscle stem-cell population, can differentiate into fat cells. We hypothesized that satellite cells from the rotator cuff have greater intrinsic adipogenic differentiation potential than do gastrocnemius satellite cells, and this difference is due to variations in epigenetic imprinting between the cells. METHODS:Satellite cells from gastrocnemius and rotator cuff muscles of mice were cultured in adipogenic media, and t

SUBMITTER: Schubert MF 

PROVIDER: S-EPMC6791507 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature