Biphenotypic Differentiation of Pancreatic Cancer in 3-Dimensional Culture.

ABSTRACT:

Objective

Pancreatic ductal adenocarcinoma (PDAC) is the third most common cause of cancer death in the United States. Improved characterized models of PDAC are needed for drug screening.

Methods

We grew 4 established pancreatic cancer cell lines in hanging drop cultures to produce spheroids. We also grew organoids from explanted xenografted PDAC and surgically resected primary PDAC. We performed transmission and scanning electron microscopy and compared findings with those of the normal pancreatic duct. We also performed single-cell cloning to determine the potential options for differentiation.

Results

Spheroids contained tight junctions and desmosomes but lacked zymogen granules, as expected. The former features were present in normal pancreatic duct but absent fro

SUBMITTER: Matsushita Y 

PROVIDER: S-EPMC6791773 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature