Experimental challenge with bovine respiratory syncytial virus in dairy calves: bronchial lymph node transcriptome response.

ABSTRACT: Bovine Respiratory Disease (BRD) is the leading cause of mortality in calves. The objective of this study was to examine the response of the host's bronchial lymph node transcriptome to Bovine Respiratory Syncytial Virus (BRSV) in a controlled viral challenge. Holstein-Friesian calves were either inoculated with virus (10^3.5 TCID₅₀/ml × 15 ml) (n = 12) or mock challenged with phosphate buffered saline (n = 6). Clinical signs were scored daily and blood was collected for haematology counts, until euthanasia at day 7 post-challenge. RNA was extracted and sequenced (75 bp paired-end) from bronchial lymph nodes. Sequence reads were aligned to the UMD3.1 bovine reference genome and differential gene expression analysis was performed using EdgeR. There was a clear separatio

SUBMITTER: Johnston D 

PROVIDER: S-EPMC6791843 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature