Transcriptional activation of CBFβ by CDK11^p110 is necessary to promote osteosarcoma cell proliferation.

ABSTRACT:

Background

Aberrant expression of cyclin-dependent protein kinases (CDK) is a hallmark of cancer. CDK11 plays a crucial role in cancer cell growth and proliferation. However, the molecular mechanisms of CDK11 and CDK11 transcriptionally regulated genes are largely unknown.

Methods

In this study, we performed a global transcriptional analysis using gene array technology to investigate the transcriptional role of CDK11 in osteosarcoma. The promoter luciferase assay, chromatin immunoprecipitation assay, and Gel Shift assay were used to identify direct transcriptional targets of CDK11. Clinical relevance and function of core-binding factor subunit beta (CBFβ) were further accessed in osteosarcoma.

Results

We identified a transcriptional role of protein-DNA interaction for

SUBMITTER: Feng Y 

PROVIDER: S-EPMC6792216 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature