Transfection of Culicoides sonorensis biting midge cell lines with Wolbachia pipientis.

ABSTRACT:

Background

Biting midges of the genus Culicoides vector multiple veterinary pathogens and are difficult to control. Endosymbionts particularly Wolbachia pipientis may offer an alternative to control populations of Culicoides and/or impact disease transmission in the form of population suppression or replacement strategies.

Methods

Culicoides sonorensis cell lines were transfected with a Wolbachia infection using a modified shell vial technique. Infections were confirmed using PCR and cell localization using fluorescent in situ hybridization (FISH). The stability of Wolbachia infections and density was determined by qPCR. qPCR was also used to examine immune genes in the IMD, Toll and JACK/STAT pathways to determine if Wolbachia were associated with an immune response in infe

SUBMITTER: Ghosh A 

PROVIDER: S-EPMC6792224 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature