Use of fusion transcription factors to reprogram cellulase transcription and enable efficient cellulase production in Trichoderma reesei.

ABSTRACT:

Background

Trichoderma reesei is widely used for cellulase production and accepted as an example for cellulase research. Cre1-mediated carbon catabolite repression (CCR) can significantly inhibit the transcription of cellulase genes during cellulase fermentation in T. reesei. Early efforts have been undertaken to modify Cre1 for the release of CCR; however, this approach leads to arrested hyphal growth and decreased biomass accumulation, which negatively affects cellulase production.

Results

In this study, novel fusion transcription factors (fTFs) were designed to release or attenuate CCR inhibition in cellulase transcription, while Cre1 was left intact to maintain normal hyphal growth. Four designed fTFs were introduced into the T. reesei

SUBMITTER: Wang F 

PROVIDER: S-EPMC6792246 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature