A single-cell genome reveals diplonemid-like ancestry of kinetoplastid mitochondrial gene structure.

ABSTRACT: Euglenozoa comprises euglenids, kinetoplastids, and diplonemids, with each group exhibiting different and highly unusual mitochondrial genome organizations. Although they are sister groups, kinetoplastids and diplonemids have very distinct mitochondrial genome architectures, requiring widespread insertion/deletion RNA editing and extensive trans-splicing, respectively, in order to generate functional transcripts. The evolutionary history by which these differing processes arose remains unclear. Using single-cell genomics, followed by small sub unit ribosomal DNA and multigene phylogenies, we identified an isolated marine cell that branches on phylogenetic trees as a sister to known kinetoplastids. Analysis of single-cell amplified genomic material identified multiple mitochondrial g

SUBMITTER: Wideman JG 

PROVIDER: S-EPMC6792441 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature