Complete mitochondrial genomes from transcriptomes: assessing pros and cons of data mining for assembling new mitogenomes.

ABSTRACT: Thousands of eukaryotes transcriptomes have been generated, mainly to investigate nuclear genes expression, and the amount of available data is constantly increasing. A neglected but promising use of this large amount of data is to assemble organelle genomes. To assess the reliability of this approach, we attempted to reconstruct complete mitochondrial genomes from RNA-Seq experiments of Reticulitermes termite species, for which transcriptomes and conspecific mitogenomes are available. We successfully assembled complete molecules, although a few gaps corresponding to tRNAs had to be filled manually. We also reconstructed, for the first time, the mitogenome of Reticulitermes banyulensis. The accuracy and completeness of mitogenomes reconstruction appeared independent from transcriptome size

SUBMITTER: Forni G 

PROVIDER: S-EPMC6794255 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature