Synthesis and Enzymatic Incorporation of a Responsive Ribonucleoside Probe That Enables Quantitative Detection of Metallo-Base Pairs.

ABSTRACT: Synthesis of a highly responsive fluorescent ribonucleoside analogue based on a 5-methoxybenzofuran uracil core, enzymatic incorporation of its triphosphate substrate into RNA transcripts, and its utility in the specific detection and estimation of Hg²⁺-ion-mediated metallo-base pair formation in DNA-RNA and RNA-RNA duplexes are described.

SUBMITTER: Manna S 

PROVIDER: S-EPMC6794643 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature