The Effect of Maternal Obesity on Placental Cell-Free DNA Release in a Mouse Model.

ABSTRACT:

Background

The fetal fraction of cell-free DNA (cfDNA) in maternal plasma is decreased in obese women. The underlying mechanism is not well understood. The amount of cfDNA released from the placenta has not been directly examined in maternal obesity.

Objective

We sought to quantify release of cfDNA from the placenta and fetal membranes in maternal diet-induced obesity using explant cultures in an established mouse model.

Study design

C57BL6/J females were fed either 60% high-fat diet or 10% fat-matched control diet for 14 weeks prepregnancy and throughout gestation. Placentas and fetal membranes were collected on e18 and randomly allocated to time 0-, 1-, or 6-hour culture times. The CfDNA was isolated from culture media, quantified, and normalized to tissue weight.

SUBMITTER: Mhatre M 

PROVIDER: S-EPMC6794655 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature